HIV-1 p24 Antigen ELISA Kit

Product name

ELISA HIV1 p24 kit

Detection method: Colorimetric

Example type

Cell culture supernatant, Serum, Cell culture extracts, Tissue extracts, Hep plasma, EDTA plasma

Test type: Sandwich (quantitative)

Sensitivity: 1.1 pg / ml

Distance

4.69 pg / ml – 300 pg / ml

Test time: 1h 30m

Trial duration: One-step test

Species reactivity

Reacts with: Human

Product description

The HIV1 p24 ELISA Kit (ab218268) is a 90-minute single wash sandwich ELISA designed for the quantitative measurement of HIV1 p24 protein in human serum, plasma, cell culture supernatant, and cell and tissue extract samples. It uses our patented SimpleStep ELISA® technology. Quantify HIV1 p24 with a sensitivity of 1.1 pg/ml.

SimpleStep ELISA® technology employs capture antibodies conjugated with an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This sandwich ELISA approach allows for the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the simple steps ELISA protocol summary in the imaging section for more details. Our ELISA SimpleStep technology offers several benefits:

  • Single wash protocol reduces assay time to 90 minutes or less
  • High sensitivity, specificity and reproducibility of superior antibodies
  • Fully validated in biological samples
  • 96-well plate that can be broken into 12 x 8-well strips

ASSAY SPECIFICITY This kit recognizes both native and recombinant HIV-1 p24 protein in serum, plasma with EDTA and heparin, cell culture supernatant, and cell and tissue extracts only. Urine, saliva, and milk samples have not been tested with this kit.

CROSS-REACTIVITY Recombinant HIV-1 Gag protein was prepared at 50 ng/ml and 1 ng / ml and analyzed for cross-reactivity. A cross-reactivity of 1% was observed.

Notes

The HIV1 p24 (capsid) protein is essential for HIV-1 viral replication and for HIV-1 infection of non-dividing cells. The HIV1 p24 proteins form a viral capsid that encapsulates the HIV1 genomic RNA. The HIV1 p24 concentration in host plasma is commonly used as an indicator of viral load. Following viral infection, the development of anti-HIV1 p24 host humoral responses leads to the formation of immune complexes and a reduction in the concentration of free circulating HIV1 p24.

Abcam has not applied for and does not intend to apply for REACH Authorization for the use of products by customers containing substances on the European Authorization List (Annex XIV). It is the responsibility of our clients to verify the need to apply the REACH Authorization, and any other relevant authorization, for the intended uses.

Platform

Precoated Microplate (12 x 8-well strips)

Properties

Storage instructions

Store at + 4 ° C. Consult protocols.

Relevance

HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, proteins form membrane associations and self-association that ultimately result in the budding of an immature virion from the infected cell. The gag precursors also function during viral assembly to selectively join and package two more strands of genomic RNA. The capsid protein p24 probably forms the conical nucleus of the virus that encapsulates the RNA-genomic nucleocapsid complex.

Cell location: Membrane

Alternative names

  • California
  • Capsid protein p24
  • HIV-1 p24 gag

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